Conference Program

Sunday – October 7, 2018
4:00-6:00 Registration & Poster Display
6:00-08:00 Welcome Reception
10:00-3:00 Workshops

How much virus reduction do we need for wastewater intended for recycling?

Guidelines and standards for virus removal for recycled water. How much credit do we give for treatment processes?

–Channah Rock

Virus removal by conventional wastewater treatment as monitored by qPCR

–Masaaki Kitajima

Virus removal by RO membranes

–Gertjan Medema


–Charles Gerba


Measurement of virus infectivity - where do we stand?

Brief overview of methods for measure of virus infectivity using cell culture

–Charles Gerba

Approaches to measure of norovirus in cells – what is needed for application to virus detection in the environment?

–Jan Vinjé

Molecular methods for measure of virus infectivity

–Jason Torres


–Charles Gerba

Monday – October 8, 2018
8:30-9:30 Opening Ceremony

Opening Remarks: Professor Morteza Abbaszadegan

Arizona State University – USA

Welcome Speech: Professor Charles Gerba

University of Arizona – USA

Welcome remarks: Dr. Ricardo Santos

President of ISFEV – Portugal


Plenary Lecture: Professor Albert Bosch – Hep A is here to stay

– University of Barcelona

Session 1

Viruses in Food & Water & their Public Health Impacts


Prevalence of human enteric viruses in shellfish from Galicia (NW Spain): A 20-year perspective

–Jesús Romalde, Enrique Rivadulla, Miguel Varela, Juan Barja


The European Union Reference Laboratory for foodborne viruses

–Magnus Simonsson


Occurrence and diversity of human enteroviruses circulating in Luxembourg

–Leslie Ogorzaly


Human sapovirus in wastewaters from Tunisia

– Miguel Varela, Imen Ouardani, Enrique Rivadulla, Tsuyoshi Kato, Syunsuke Kadoya, Mahjoub Aouni, Daisuke Sano, Jesús Romalde

10:30-10:50 Coffee Break

Evaluation and implementation of mitigation strategies for viral pathogens in the berry supply chain

– Francoise Julien-Javaux, Matteo Campagnoli, Zijin Zhou, Dan Li, Mieke Uyettendaele, Sophie Butot, Sophie Zuber


Inactivation of HAV, MNV and MS2 on onions, raisins and blueberries during mild drying processes

– Sophie Butot, T. Putallaz, M. Moser, S. Zuber


Green tea extract to control enteric viruses in food

– Irene Falcó, Maria Jose Fabra, Walter Randazzo, Laura Gómez-Mascaraque, Rosa Aznar, Amparo López-Rubio, Gloria Sánchez


Assessment of novel approaches to investigate norovirus persistency in the aquatic environment

– Kata Farkas, Tim Fitzmaurice, Ian Goodfellow, James McDonald, Shelagh Malham, Davey Jones


Verification of bag-mediated filtration system V2

– Christine Fagnant-Sperati, Alexandra Kossik, Nicolette Zhou, Evans Komen, Benlick Mwangi, James Nyangao, Jaffer Hassan, A. Chepkurui, Byron Ockerman, Geneva. Goldwood, Michael Eisenstein, Jeffry Shirai, Nicola Beck, Igor Novosselov, David Boyle, Peter Borus, John Scott Meschke

12:05-01:00 Lunch Break
1:00–2:15 Poster Session
Session 2

Analytical Methods for Virus Detection & Quantification


Keynote speaker: Gertjan Medema – KWR, Netherlands

The use of natural viruses in surface water for the verification of integrity of RO membranes

– Luc M Hornstra, Leo Heijnen, Tania Rodrigues da Silva , Bastiaan Blankert, Erwin Beerendonk, Emile Cornelissen, Gertjan Medema


Comparison between RT droplet digital PCR and RT real-time PCR for quantification of noroviruses in oysters

– Sofia Persson


Infectivity tests coupled with metagenomic analysis reveal the presence of several infectious viruses in wastewaters

– Katarina Bačnik, Denis Kutnjak, Anja Pecman, Ion Gutierrez Aguirre, Nataša Mehle, Magda Tušek Žnidarič, Maja Ravnikar


Development of continuous flow incubation system to detect single infective coliphage from a large volume water sample

– Akihiko Hata, Shotaro Torii, Hiro Katayama

3:30-3:50 Coffee Break

Application of nanopore MinION for rapid viral pathogen detection in aquatic environments

– Pan Ji, Joan Rose, William Van Bonn


Development of a Real-Time Cell Analysis (RTCA) method as a fast and accurate method for detecting infectious particles of hepatitis A virus

– Samuel Lebourgeois, Audrey Fraisse, Catherine Hennechart-Collette, Laurent Guillier, Sylvie Perelle Sandra Martin-Latil


Evaluation of a modified direct lysis method for detection of foodborne viruses on various fruit and vegetable

– Sheikh Rajiuddin, A Anna Charlotte Schultz


Targeted Amplicon Deep Sequencing Analysis: Diversity of adenovirus, papillomavirus and enterovirus in raw sewage

– Sandra Martínez-Puchol, Marta Rusiñol, Xavier Fernández-Cassi, Natàlia Timoneda, Rosina Girones, Sílvia Bofill-Mas


Viral processing of spiked human norovirus GI and GII influences viral persistence during disinfection of water

– Kellogg Schwab, Nathan Dunkin


Specific detection of negative-strand viral RNA for determining norovirus infectivity

– Masaaki Kitajima, Moegi Ohama, Daisuke Sano, Satoshi Okabe

Tuesday – October 9, 2018
Session 3

Risk Assessment & Viral Occurrence


Keynote speaker: Kyle Bibby – University of Notre Dame, USA

Geographic distribution of cross-assembly phage in sewage

– Kyle Bibby, Katherine Crank, Zhenyu Wu, Xiang Li, Giuseppina La Rosa


Will we ever be able to agree a single dose response model for norovirus or is such a model merely a myth

– Paul Hunter


Addressing research gaps to transition norovirus cell culture assays from research to environmental applications

– Timothy Straub


Assessment of viral pathogens, their indicators, and the applicability of quantitative microbial risk assessment in irrigation waters

– Jennifer Pearce-Walker


Health risks associated with playgrounds: Decay of human enteroviruses in natural sand and fine gravel

– Silvia Monteiro, Ricardo Santos


Effectiveness of sodium deoxycholate pre-treatment to improve viability RT-qPCR for discrimination of inactivated viruses in drinking water

– Vu Duc Canh, Hiroyuki Katayama, Hiroaki Furumai


Surveillance of enteric viruses in sewage reveals high viral diversity and synchronous time patterns with number of hospital patients infected with the viruses identified

– Hao Wang, Ellen Magnil, Julianna Neyvaldt, Lucica Enacha, Ann Mattsson, Olof Bergstedt, Helene Norder

10:30-10:50 Coffee Break
Session 4

Viral Structure, Stability, Fate & Transport


Keynote speaker: Brooke Mayer – Marquette University, USA

The role of virus structure in removal and inactivation of health-related viruses


Structural characterization of thermoresistant enteroviruses

– Simon Meister, Alessio Prunotto, Matteo Dal Peraro, Tamar Kohn


Physisorption and chemisorption of T4 bacteriophages on amino functionalized silica particles

– Stephanie Bone, Absar Alum, Jasmina Markovski, Kiril Hristovski, Edo Bar-Zeev, Yair Kaufman, Morteza Abbaszadegan, Francois Perreault


Interaction between human noroviruses and Acanthamoeba castellanii: influence on the viral persistence in water environment

– Benoit Prevost


Enterovirus interaction with organic matter: Impact on virus stability

– Prunelle Waldman, Françoise Lucas, Laurent Moulin, Sébastien Wurtzer

12:20-1:10 Lunch Break
1:00–2:15 Poster Session
Session 5

Emerging Technologies for Viral Disinfection & Treatmen

2:30 – 3:00

Keynote speaker: Daisuke Sano – Tohoku University, Japan

Inactivation and removal of viruses: is this a matter of virus evolution?

3:00 – 3:15

A review of the current state of UV virus inactivation

– George Diefenthal


Virus inactivation by ozone in natural matrices: potential proxies for viral inactivation

– Camille Wolf

3:30 – 3:45

Inactivation mechanism of rotavirus, human norovirus, and human norovirus surrogate Tulane virus by food sanitizers

– Miyu Fuzawa

3:45-4:05 Coffee Break

A RTCA-based assay as an innovative approach for thermal inactivation studies of Hepatitis A virus (HAV)

– Audrey Fraisse, Catherine Hennechart-Collette, Laurent Guillier, Sylvie Perelle and Sandra Martin-Latil


Impact of intermittent operation on virus removal by residential RO membrane

– Shotaro Torii, T. Hashimoto, T. Do, Hiroaki Furumai, Hiroyuki Katayama

4:35 – 4:50

Removal efficiency of indigenous F-specific RNA phages in inline coagulation and micro-filtration system affected by different coagulation characteristics

– Midori Yasui, T. Yonetani, L. Ikner, Hiroaki Furumai, Hiroyuki Katayama


Synergistic effects of TiO2 and PAA for inactivation of viruses

– Morteza Abbaszadegan, Majid Nikougoftar Zarif, Absar Alum

Gala Dinner
Wednesday – October 10, 2018
Session 6

Virus Outbreaks, Epidemiology, & Source Tracking


Keynote speaker: Jan Vinjé – Centers for Disease Control & Prevention, USA

Successful replication of human norovirus in human intestinal enteroids as a model to measure virus inactivation

– Jan Vinjé, Esther Morantz, Hannah Browne, Khalil Ettayebi, Xi-Lei Zeng, Robert Atmar, Mary Estes, Veronica Costantini


Two drinking water outbreaks caused by sapovirus in Finland

– Ari Kauppinen, Tarja Pitkänen, Haider Al-Hello, Leena Maunula, Ruska Rimhanen-Finne, Ilkka Miettinen


Enhanced poliovirus detection in Pakistan environmental samples using the bag-mediated filtration system

– Nicolette Zhou, Christine Fagnant-Sperati, Jeffry Shirai, Salmaan Sharif, Muhammed Salman, Sohail Zaidi, Rahim Agha, Lubna Rehman, Jaffer Hussain, Shahzad Shaukat, Masroor Alam, Adnan Khurshid, Ghulam Mujtaba, Rana Muhammed Safdar, Abdirahman Mahamud, Jamal Ahmed, Sadaf Khan, Alexandra L. Kossik, Nicola K. Beck, Humayun Asghar, Ananda Bandyopadhyay, David Boyle, Scott Meschke

9:30 – 9:45

Prevalence of norovirus in produce sold at retail in the United Kingdom

– Martin D'Agostino

9:45 – 10:00

Towards routine analysis of infectious norovirus in bivalve shellfish

– David Walker

3:45-4:05 Coffee Break
Session 7

Emerging Issues


Keynote speaker: Rosina Girones – University of Barcelona, Spain

Viral populations and quality control of irrigation waters

– Marta Rusiñol, Xavi Fernandez-Cassi, Natalia Timoneda, Eloy Gonzales-Gustavson, Josep Francesc Abril, Gertjan Medema, Calvo M, Sandra Martínez-Puchol, Sylvia Bofill-Mas, Rosina Gironés


Inactivation of porcine viruses in the production process of spray dried porcine plasma

– Marcel Hulst, Renate Hakze van der Honing, Meindert Pelser, Martijn Fox Lourens Heres, Wim H. M. van der Poel


Determining viral genome functionality loss after various disinfection treatments using a novel transfection-based most probable number assay

– Jason Torrey

11:30 – 11:45

Suitability of bacteriophages as surrogates for virus mitigation by electrocoagulation

– Joe Heffron, Brooke Mayer


Assessment of bacteriophage MS2 removal and contaminant reduction by biosand filter

– Chunhwa Jang


Identification of bacterial genes for producing norovirus-binding glycoconjugate

– Mohan Amarasiri, Peiyi Yang, Masaaki Kitajima, Yasuhiro Kasahara, Satoshi Okabe, Daisuke Sano


Challenges of infectious hepatitis E virus detection (in food products)

– Willemijn Lodder, Saskia Rutjes

12:30-1:00 Closing Ceremony
Poster Presentation – October 8 & 9

Poster setup during Suncday Registration and Monday Morning

Poster display on Monday and Tuesday, Arizona Ballroom, MU

Poster Number

Session 1: Viruses in Food & Water & their Public Health Impacts


Detection of foodborne enteric viruses in Irish oysters intended for human consumption

– Amy Heather Fitzpatrick, Sinéad Keaveney, Agnieszka Rupnik, Leon Devilly, James Fahy, William Doré


Molecular epidemiology of Hepatitis E Virus (HEV) in human and swine populations in Ireland

– Charlene Bennett, Conor Davin, Linda Dunford, Suzie Coughlan, Cillian De Gascun, Joanne O’Gorman


Sapovirus and Aichi virus associated to imported shellfish from developing countries

– Enrique Rivadulla, Miguel F Varela, Antía Loureiro, Jesús L Romalde


Human health risk associated with CrAssphage gene marker in fresh untreated sewage- and tertiary effluent- contaminated recreational water

– Kerry Hamilton, Warish Ahmed, Aldo Lobos, Valerie J. Harwood


Rotavirus uptake rate and decay rate for arugula (Eruca sativa) internalization determined for quantitative microbial risk assessment

– Miyu Fuzawa


Acanthamoebidae and Mimiviridae Sequences Detected in the Feces of Puerto Rican Pre-Columbian Cultures

– Rosana Wiscovitch Russo


Detection of noroviruses and hepatitis E virus in pork meat product chain

– Petra Vasickova, M. Kubankova, P. Mikel


Quantitative Detection of Human Adenovirus in wastewater treatment plant influencing the Mississippi River in Louisiana

– Samendra Sherchan


Analysis of fecal source of contamination using viral indicators in Santa Lucia and Uruguay rivers

– Viviana Bortagaray

Poster Number

Session 2: Analytical Methods for Virus Detection & Quantification


Bag-mediated filtration system v2: Modifications and improvements

– Alexandra Kossik, Christine S. Fagnant-Sperati, Jeffry H. Shirai , Nicolette A. Zhou , Bethel A. Demeke, Nicola K. Beck, Geneva Goldwood, Michael Eisenstein, Byron Ockerman, Igor Novosselov, David S. Boyle, J. Scott Meschke


Metagenomics for the detection and characterization of HEV in water, food and clinical samples

– Sílvia Bofill-Mas, Xavier Fernández-Cassi, Eloy Gonzales-Gustavson, Natàlia Timoneda, Marta Rusiñol, Sandra Martínez-Puchol, Josep J. Abril, Rosina Girones


Virwatest: a method for detection of viruses in water samples in the point-of-use

– David Aguado, Eva Forés, Laura Guerrero, Marta Rusiñol, Sandra Martínez-Puchol, Francesc Codony, Rosina Girones, Sílvia Bofill-Mas


The utility of flow cytometry in water reuse applications

– Nicole Rockey, Heather N. Bischel, Lutgarde Raskin, Tamar Kohn, Brian Pecson, Krista R. Wigginton


Synthetic-long read next generation sequencing techniques for poliovirus identification in environmental samples

– Nicolette Zhou, Andrew Fields, Nicola Beck, Andrew Adey, J. Scott Meschke


Developing a method to specifically and simultaneously measure the concentration of infective enterovirus serotypes in environmental samples

– Odile Larivé, Jade Brandani, Tamar Kohn


Process of acquisition of UKAS accreditation for detection of norovirus GI&GII and hepatitis A in berry fruit and leafy greens

– Olivera Maksimovic, Martin D’Agostino


Adaptation of a cationic bead norovirus extraction protocol to berry type matrices

– Philippe Raymond


Human Intestinal Enteroids as a Model for Enteric Virus Infection

– Samantha Wales, Zhihui Yang, Diana Ngo, Michael Kulka


New insights for optimizing molecular detection of infectious viruses

– Sebastien Wurtzer


Assessment of pathogens in treated wastewater intended for crop irrigation

– Walter Betancourt, Christina Morrison, Daisy Hurtado, Libin Zhu, Sadhana Ravishankar, Natalie Brassill, Channah Rock, Luisa Ikner, Charles Gerba


Evaluating the performance of conventional and new viral indicator assays for human pathogenic enteric viruses in food and water matrices

– Walter Betancourt, Christina Morrison, Daisy Hurtado, Charles Gerba


Hepatitis E Virus in the Aquatic Environment

– Samantha Treagus

Poster Number

Session 3: Risk Assessment & Viral Occurrence


Hepatitis E in Irish pork production

– Conor Davin, Louise Britton, Eoin Ryan, Charlene Bennett, John Moriarty, Edgar-Garcia Manzanilla, Lorna O'Brien, Suzie Coughlan, Joanne O’Gorman


Estimating the effect of a quaternary ammonium hand sanitizer on norovirus infection risk

– Amanda Wilson

Poster Number

Session 4: Viral Structure, Stability, Fate & Transport


Reduction of Aichi virus by blueberry proanthocyanidins in model foods and under simulated gastric conditions

– S. Joshi, A. Howell, and D. H. D'Souza


Hepatitis A virus inactivation by curcumin and heat

– M. Patwardhan and D. H. D'Souza


Persistence of norovirus in berry smoothies

– Milla Pitkänen, Agathe Laudet, Leena Maunula


Two-year monitoring of F-specific RNA bacteriophages in Orne River (France)

– Ogorzaly Leslie, Martínez-Carreras Núria, Walczak Cécile, Hissler Christophe, Cauchie Henry-Michel

Poster Number

Session 5: Emerging Technologies for Viral Disinfection & Treatment


Fate of bacteriophage during anammox side stream treatment of wastewater effluent

– Aidan Foster, Luisa Ikner, Charles Gerba, Ian Pepper


Assessing the reduction of naturally occurring viruses by soil aquifer treatment of treated wastewater effluent

– Christina Morrison


Reduction of human and plant viruses at a constructed wetland in Arizona, USA

– Eiji Haramoto, Ruriko Ito, Walter Betancourt, Charles Gerba


Strain-specific Differences in Response of Human Noroviruses to pH Challenge

– Rebecca Goulter


In-vitro and in-vivo efficacies of hand sanitizers against human norovirus

– Tadeja Lukezic


Hidrodynamic cavitation, a powerful tool for waterborne virus inactivation

– Tadeja Lukezic


Non-oxidizing agent to control viruses and other pathogens of public health significance

– Scott Yenglin, Absar Alum and Morteza Abbaszadegan

Poster Number

Session 6: Virus Outbreaks, Epidemiology & Source Tracking


Developing buffer zones for norovirus in shellfish production areas using chemical tracers, environmental microbiology and satellite remote sensing

– Carlos Campos, David Walker, Simon Kershaw, Andrew Younger, Jorge Diogène Fadini, Gary Rawle


Hepatitis A outbreaks and environmental circulation of genotype IA strains in metropolitan São Paulo, 2017-2018

– Maria Inês Sato


Foodborne viruses - molecular epidemiology in the Czech Republic

– Petra Vasickova, M. Kubankova, P. Mikel, M. Bena


Evaluation of crAssphage-based qPCR marker with human fecal indicators in Lake Pontchartrain, Louisiana

– Samendra Sherchan

Poster Number

Session 7: Emerging Issues


Algal Phages – a Threat to the Health of Algal Production Operation for Food and Biofuel Application

– Absar Alum, Kyle Kraft, Morteza Abbaszadegan


Whole genome sequencing reveals prophage sequences present in Escherichia coli isolated from pristine environments

– Alfredo Gonzalez


Chemical Induction Followed by High-Throughput Sequencing: Prophage Presence, Host Range and Promiscuity as Possible Roles in Genome Evolution

– Yadeliz Adlin, Serrano Matos