Conference Program

Register
Sunday – October 7, 2018
4:00-6:00 Registration & Poster Display
6:00-08:00 Welcome Reception
10:00-3:00 Workshops
10:00–12:00

How much virus reduction do we need for wastewater intended for recycling?

Guidelines and standards for virus removal for recycled water. How much credit do we give for treatment processes?

–Channah Rock

Virus removal by conventional wastewater treatment as monitored by qPCR

–Masaaki Kitajima

Virus removal by RO membranes

–Gertjan Medema

Discussion

–Charles Gerba

1:00–3:00

Measurement of virus infectivity - where do we stand?

Brief overview of methods for measure of virus infectivity using cell culture

–Charles Gerba

Approaches to measure of norovirus in cells – what is needed for application to virus detection in the environment?

–Jan Vinjé

Molecular methods for measure of virus infectivity

–Jason Torres

Discussion

–Charles Gerba

Monday – October 8, 2018
8:30-9:30 Opening Ceremony

Opening Remarks: Professor Morteza Abbaszadegan

Arizona State University – USA

Welcome Speech: Professor Charles Gerba

University of Arizona – USA

Welcome remarks: Dr. Ricardo Santos

President of ISFEV – Portugal

8:45-9:30

Plenary Lecture: Professor Albert Bosch – Hep A is here to stay

– University of Barcelona

Session 1

Viruses in Food & Water & their Public Health Impacts

9:30–9:45

Prevalence of human enteric viruses in shellfish from Galicia (NW Spain): A 20-year perspective

–Jesús Romalde, Enrique Rivadulla, Miguel Varela, Juan Barja

9:45-10:00

The European Union Reference Laboratory for foodborne viruses

–Magnus Simonsson

10:00-10:15

Occurrence and diversity of human enteroviruses circulating in Luxembourg

–Leslie Ogorzaly

10:15–10:30

Human sapovirus in wastewaters from Tunisia

– Miguel Varela, Imen Ouardani, Enrique Rivadulla, Tsuyoshi Kato, Syunsuke Kadoya, Mahjoub Aouni, Daisuke Sano, Jesús Romalde

10:30-10:50 Coffee Break
10:50–11:05

Evaluation and implementation of mitigation strategies for viral pathogens in the berry supply chain

– Francoise Julien-Javaux, Matteo Campagnoli, Zijin Zhou, Dan Li, Mieke Uyettendaele, Sophie Butot, Sophie Zuber

11:05–11:20

Inactivation of HAV, MNV and MS2 on onions, raisins and blueberries during mild drying processes

– Sophie Butot, T. Putallaz, M. Moser, S. Zuber

11:20–11:35

Green tea extract to control enteric viruses in food

– Irene Falcó, Maria Jose Fabra, Walter Randazzo, Laura Gómez-Mascaraque, Rosa Aznar, Amparo López-Rubio, Gloria Sánchez

11:35–11:50

Assessment of novel approaches to investigate norovirus persistency in the aquatic environment

– Kata Farkas, Tim Fitzmaurice, Ian Goodfellow, James McDonald, Shelagh Malham, Davey Jones

11:50–12:05

Verification of bag-mediated filtration system V2

– Christine Fagnant-Sperati, Alexandra Kossik, Nicolette Zhou, Evans Komen, Benlick Mwangi, James Nyangao, Jaffer Hassan, A. Chepkurui, Byron Ockerman, Geneva. Goldwood, Michael Eisenstein, Jeffry Shirai, Nicola Beck, Igor Novosselov, David Boyle, Peter Borus, John Scott Meschke

12:05-01:00 Lunch Break
1:00–2:15 Poster Session
Session 2

Analytical Methods for Virus Detection & Quantification

2:15–2:45

Keynote speaker: Gertjan Medema – KWR, Netherlands

The use of natural viruses in surface water for the verification of integrity of RO membranes

– Luc M Hornstra, Leo Heijnen, Tania Rodrigues da Silva , Bastiaan Blankert, Erwin Beerendonk, Emile Cornelissen, Gertjan Medema

2:45–3:00

Comparison between RT droplet digital PCR and RT real-time PCR for quantification of noroviruses in oysters

– Sofia Persson

3:00–3:15

Infectivity tests coupled with metagenomic analysis reveal the presence of several infectious viruses in wastewaters

– Katarina Bačnik, Denis Kutnjak, Anja Pecman, Ion Gutierrez Aguirre, Nataša Mehle, Magda Tušek Žnidarič, Maja Ravnikar

3:15–3:30

Development of continuous flow incubation system to detect single infective coliphage from a large volume water sample

– Akihiko Hata, Shotaro Torii, Hiro Katayama

3:30-3:50 Coffee Break
3:50–4:05

Application of nanopore MinION for rapid viral pathogen detection in aquatic environments

– Pan Ji, Joan Rose, William Van Bonn

4:05–4:20

Development of a Real-Time Cell Analysis (RTCA) method as a fast and accurate method for detecting infectious particles of hepatitis A virus

– Samuel Lebourgeois, Audrey Fraisse, Catherine Hennechart-Collette, Laurent Guillier, Sylvie Perelle Sandra Martin-Latil

4:20-4:35

Evaluation of a modified direct lysis method for detection of foodborne viruses on various fruit and vegetable

– Sheikh Rajiuddin, A Anna Charlotte Schultz

4:35-4:50

Targeted Amplicon Deep Sequencing Analysis: Diversity of adenovirus, papillomavirus and enterovirus in raw sewage

– Sandra Martínez-Puchol, Marta Rusiñol, Xavier Fernández-Cassi, Natàlia Timoneda, Rosina Girones, Sílvia Bofill-Mas

4:50-5:05

Viral processing of spiked human norovirus GI and GII influences viral persistence during disinfection of water

– Kellogg Schwab, Nathan Dunkin

5:05-5:20

Specific detection of negative-strand viral RNA for determining norovirus infectivity

– Masaaki Kitajima, Moegi Ohama, Daisuke Sano, Satoshi Okabe

Tuesday – October 9, 2018
Session 3

Risk Assessment & Viral Occurrence

8:30–9:00

Keynote speaker: Kyle Bibby – University of Notre Dame, USA

Geographic distribution of cross-assembly phage in sewage

– Kyle Bibby, Katherine Crank, Zhenyu Wu, Xiang Li, Giuseppina La Rosa

9:00-9:15

Will we ever be able to agree a single dose response model for norovirus or is such a model merely a myth

– Paul Hunter

9:15-9:30

Addressing research gaps to transition norovirus cell culture assays from research to environmental applications

– Timothy Straub

9:30-9:45

Assessment of viral pathogens, their indicators, and the applicability of quantitative microbial risk assessment in irrigation waters

– Jennifer Pearce-Walker

9:45-10:00

Health risks associated with playgrounds: Decay of human enteroviruses in natural sand and fine gravel

– Silvia Monteiro, Ricardo Santos

10:00-10:15

Effectiveness of sodium deoxycholate pre-treatment to improve viability RT-qPCR for discrimination of inactivated viruses in drinking water

– Vu Duc Canh, Hiroyuki Katayama, Hiroaki Furumai

10:15-10:30

Surveillance of enteric viruses in sewage reveals high viral diversity and synchronous time patterns with number of hospital patients infected with the viruses identified

– Hao Wang, Ellen Magnil, Julianna Neyvaldt, Lucica Enacha, Ann Mattsson, Olof Bergstedt, Helene Norder

10:30-10:50 Coffee Break
Session 4

Viral Structure, Stability, Fate & Transport

10:50-11:20

Keynote speaker: Brooke Mayer – Marquette University, USA

The role of virus structure in removal and inactivation of health-related viruses

11:20-11:35

Structural characterization of thermoresistant enteroviruses

– Simon Meister, Alessio Prunotto, Matteo Dal Peraro, Tamar Kohn

11:35-11:50

Physisorption and chemisorption of T4 bacteriophages on amino functionalized silica particles

– Stephanie Bone, Absar Alum, Jasmina Markovski, Kiril Hristovski, Edo Bar-Zeev, Yair Kaufman, Morteza Abbaszadegan, Francois Perreault

11:50-12:05

Interaction between human noroviruses and Acanthamoeba castellanii: influence on the viral persistence in water environment

– Benoit Prevost

12:05-12:20

Enterovirus interaction with organic matter: Impact on virus stability

– Prunelle Waldman, Françoise Lucas, Laurent Moulin, Sébastien Wurtzer

12:20-1:10 Lunch Break
1:00–2:15 Poster Session
Session 5

Emerging Technologies for Viral Disinfection & Treatmen

2:30 – 3:00

Keynote speaker: Daisuke Sano – Tohoku University, Japan

Inactivation and removal of viruses: is this a matter of virus evolution?

3:00 – 3:15

A review of the current state of UV virus inactivation

– George Diefenthal

3:15-3:30

Virus inactivation by ozone in natural matrices: potential proxies for viral inactivation

– Camille Wolf

3:30 – 3:45

Inactivation mechanism of rotavirus, human norovirus, and human norovirus surrogate Tulane virus by food sanitizers

– Miyu Fuzawa

3:45-4:05 Coffee Break
4:05-4:20

A RTCA-based assay as an innovative approach for thermal inactivation studies of Hepatitis A virus (HAV)

– Audrey Fraisse, Catherine Hennechart-Collette, Laurent Guillier, Sylvie Perelle and Sandra Martin-Latil

4:20-4:35

Impact of intermittent operation on virus removal by residential RO membrane

– Shotaro Torii, T. Hashimoto, T. Do, Hiroaki Furumai, Hiroyuki Katayama

4:35 – 4:50

Removal efficiency of indigenous F-specific RNA phages in inline coagulation and micro-filtration system affected by different coagulation characteristics

– Midori Yasui, T. Yonetani, L. Ikner, Hiroaki Furumai, Hiroyuki Katayama

4:50-5:05

Synergistic effects of TiO2 and PAA for inactivation of viruses

– Morteza Abbaszadegan, Majid Nikougoftar Zarif, Absar Alum

Gala Dinner
Wednesday – October 10, 2018
Session 6

Virus Outbreaks, Epidemiology, & Source Tracking

8:30-9:00

Keynote speaker: Jan Vinjé – Centers for Disease Control & Prevention, USA

Successful replication of human norovirus in human intestinal enteroids as a model to measure virus inactivation

– Jan Vinjé, Esther Morantz, Hannah Browne, Khalil Ettayebi, Xi-Lei Zeng, Robert Atmar, Mary Estes, Veronica Costantini

9:00-9:15

Two drinking water outbreaks caused by sapovirus in Finland

– Ari Kauppinen, Tarja Pitkänen, Haider Al-Hello, Leena Maunula, Ruska Rimhanen-Finne, Ilkka Miettinen

9:15-9:30

Enhanced poliovirus detection in Pakistan environmental samples using the bag-mediated filtration system

– Nicolette Zhou, Christine Fagnant-Sperati, Jeffry Shirai, Salmaan Sharif, Muhammed Salman, Sohail Zaidi, Rahim Agha, Lubna Rehman, Jaffer Hussain, Shahzad Shaukat, Masroor Alam, Adnan Khurshid, Ghulam Mujtaba, Rana Muhammed Safdar, Abdirahman Mahamud, Jamal Ahmed, Sadaf Khan, Alexandra L. Kossik, Nicola K. Beck, Humayun Asghar, Ananda Bandyopadhyay, David Boyle, Scott Meschke

9:30 – 9:45

Prevalence of norovirus in produce sold at retail in the United Kingdom

– Martin D'Agostino

9:45 – 10:00

Towards routine analysis of infectious norovirus in bivalve shellfish

– David Walker

3:45-4:05 Coffee Break
Session 7

Emerging Issues

10:30-11:00

Keynote speaker: Rosina Girones – University of Barcelona, Spain

Viral populations and quality control of irrigation waters

– Marta Rusiñol, Xavi Fernandez-Cassi, Natalia Timoneda, Eloy Gonzales-Gustavson, Josep Francesc Abril, Gertjan Medema, Calvo M, Sandra Martínez-Puchol, Sylvia Bofill-Mas, Rosina Gironés

11:00-11:15

Inactivation of porcine viruses in the production process of spray dried porcine plasma

– Marcel Hulst, Renate Hakze van der Honing, Meindert Pelser, Martijn Fox Lourens Heres, Wim H. M. van der Poel

11:15-11:30

Determining viral genome functionality loss after various disinfection treatments using a novel transfection-based most probable number assay

– Jason Torrey

11:30 – 11:45

Suitability of bacteriophages as surrogates for virus mitigation by electrocoagulation

– Joe Heffron, Brooke Mayer

11:45-12:00

Assessment of bacteriophage MS2 removal and contaminant reduction by biosand filter

– Chunhwa Jang

12:00-12:15

Identification of bacterial genes for producing norovirus-binding glycoconjugate

– Mohan Amarasiri, Peiyi Yang, Masaaki Kitajima, Yasuhiro Kasahara, Satoshi Okabe, Daisuke Sano

12:15-12:30

Challenges of infectious hepatitis E virus detection (in food products)

– Willemijn Lodder, Saskia Rutjes

12:30-1:00 Closing Ceremony
Poster Presentation – October 8 & 9

Poster setup during Suncday Registration and Monday Morning

Poster display on Monday and Tuesday, Arizona Ballroom, MU

Poster Number

Session 1: Viruses in Food & Water & their Public Health Impacts

1

Detection of foodborne enteric viruses in Irish oysters intended for human consumption

– Amy Heather Fitzpatrick, Sinéad Keaveney, Agnieszka Rupnik, Leon Devilly, James Fahy, William Doré

2

Molecular epidemiology of Hepatitis E Virus (HEV) in human and swine populations in Ireland

– Charlene Bennett, Conor Davin, Linda Dunford, Suzie Coughlan, Cillian De Gascun, Joanne O’Gorman

3

Sapovirus and Aichi virus associated to imported shellfish from developing countries

– Enrique Rivadulla, Miguel F Varela, Antía Loureiro, Jesús L Romalde

4

Human health risk associated with CrAssphage gene marker in fresh untreated sewage- and tertiary effluent- contaminated recreational water

– Kerry Hamilton, Warish Ahmed, Aldo Lobos, Valerie J. Harwood

5

Rotavirus uptake rate and decay rate for arugula (Eruca sativa) internalization determined for quantitative microbial risk assessment

– Miyu Fuzawa

6

Acanthamoebidae and Mimiviridae Sequences Detected in the Feces of Puerto Rican Pre-Columbian Cultures

– Rosana Wiscovitch Russo

7

Detection of noroviruses and hepatitis E virus in pork meat product chain

– Petra Vasickova, M. Kubankova, P. Mikel

8

Quantitative Detection of Human Adenovirus in wastewater treatment plant influencing the Mississippi River in Louisiana

– Samendra Sherchan

9

Analysis of fecal source of contamination using viral indicators in Santa Lucia and Uruguay rivers

– Viviana Bortagaray

Poster Number

Session 2: Analytical Methods for Virus Detection & Quantification

10

Bag-mediated filtration system v2: Modifications and improvements

– Alexandra Kossik, Christine S. Fagnant-Sperati, Jeffry H. Shirai , Nicolette A. Zhou , Bethel A. Demeke, Nicola K. Beck, Geneva Goldwood, Michael Eisenstein, Byron Ockerman, Igor Novosselov, David S. Boyle, J. Scott Meschke

11

Metagenomics for the detection and characterization of HEV in water, food and clinical samples

– Sílvia Bofill-Mas, Xavier Fernández-Cassi, Eloy Gonzales-Gustavson, Natàlia Timoneda, Marta Rusiñol, Sandra Martínez-Puchol, Josep J. Abril, Rosina Girones

12

Virwatest: a method for detection of viruses in water samples in the point-of-use

– David Aguado, Eva Forés, Laura Guerrero, Marta Rusiñol, Sandra Martínez-Puchol, Francesc Codony, Rosina Girones, Sílvia Bofill-Mas

13

The utility of flow cytometry in water reuse applications

– Nicole Rockey, Heather N. Bischel, Lutgarde Raskin, Tamar Kohn, Brian Pecson, Krista R. Wigginton

14

Synthetic-long read next generation sequencing techniques for poliovirus identification in environmental samples

– Nicolette Zhou, Andrew Fields, Nicola Beck, Andrew Adey, J. Scott Meschke

15

Developing a method to specifically and simultaneously measure the concentration of infective enterovirus serotypes in environmental samples

– Odile Larivé, Jade Brandani, Tamar Kohn

16

Process of acquisition of UKAS accreditation for detection of norovirus GI&GII and hepatitis A in berry fruit and leafy greens

– Olivera Maksimovic, Martin D’Agostino

17

Adaptation of a cationic bead norovirus extraction protocol to berry type matrices

– Philippe Raymond

18

Human Intestinal Enteroids as a Model for Enteric Virus Infection

– Samantha Wales, Zhihui Yang, Diana Ngo, Michael Kulka

19

New insights for optimizing molecular detection of infectious viruses

– Sebastien Wurtzer

20

Assessment of pathogens in treated wastewater intended for crop irrigation

– Walter Betancourt, Christina Morrison, Daisy Hurtado, Libin Zhu, Sadhana Ravishankar, Natalie Brassill, Channah Rock, Luisa Ikner, Charles Gerba

21

Evaluating the performance of conventional and new viral indicator assays for human pathogenic enteric viruses in food and water matrices

– Walter Betancourt, Christina Morrison, Daisy Hurtado, Charles Gerba

22

Hepatitis E Virus in the Aquatic Environment

– Samantha Treagus

Poster Number

Session 3: Risk Assessment & Viral Occurrence

23

Hepatitis E in Irish pork production

– Conor Davin, Louise Britton, Eoin Ryan, Charlene Bennett, John Moriarty, Edgar-Garcia Manzanilla, Lorna O'Brien, Suzie Coughlan, Joanne O’Gorman

24

Estimating the effect of a quaternary ammonium hand sanitizer on norovirus infection risk

– Amanda Wilson

Poster Number

Session 4: Viral Structure, Stability, Fate & Transport

25

Reduction of Aichi virus by blueberry proanthocyanidins in model foods and under simulated gastric conditions

– S. Joshi, A. Howell, and D. H. D'Souza

26

Hepatitis A virus inactivation by curcumin and heat

– M. Patwardhan and D. H. D'Souza

27

Persistence of norovirus in berry smoothies

– Milla Pitkänen, Agathe Laudet, Leena Maunula

28

Two-year monitoring of F-specific RNA bacteriophages in Orne River (France)

– Ogorzaly Leslie, Martínez-Carreras Núria, Walczak Cécile, Hissler Christophe, Cauchie Henry-Michel

Poster Number

Session 5: Emerging Technologies for Viral Disinfection & Treatment

29

Fate of bacteriophage during anammox side stream treatment of wastewater effluent

– Aidan Foster, Luisa Ikner, Charles Gerba, Ian Pepper

30

Assessing the reduction of naturally occurring viruses by soil aquifer treatment of treated wastewater effluent

– Christina Morrison

31

Reduction of human and plant viruses at a constructed wetland in Arizona, USA

– Eiji Haramoto, Ruriko Ito, Walter Betancourt, Charles Gerba

32

Strain-specific Differences in Response of Human Noroviruses to pH Challenge

– Rebecca Goulter

33

In-vitro and in-vivo efficacies of hand sanitizers against human norovirus

– Tadeja Lukezic

34

Hidrodynamic cavitation, a powerful tool for waterborne virus inactivation

– Tadeja Lukezic

35

Non-oxidizing agent to control viruses and other pathogens of public health significance

– Scott Yenglin, Absar Alum and Morteza Abbaszadegan

Poster Number

Session 6: Virus Outbreaks, Epidemiology & Source Tracking

36

Developing buffer zones for norovirus in shellfish production areas using chemical tracers, environmental microbiology and satellite remote sensing

– Carlos Campos, David Walker, Simon Kershaw, Andrew Younger, Jorge Diogène Fadini, Gary Rawle

37

Hepatitis A outbreaks and environmental circulation of genotype IA strains in metropolitan São Paulo, 2017-2018

– Maria Inês Sato

38

Foodborne viruses - molecular epidemiology in the Czech Republic

– Petra Vasickova, M. Kubankova, P. Mikel, M. Bena

39

Evaluation of crAssphage-based qPCR marker with human fecal indicators in Lake Pontchartrain, Louisiana

– Samendra Sherchan

Poster Number

Session 7: Emerging Issues

40

Algal Phages – a Threat to the Health of Algal Production Operation for Food and Biofuel Application

– Absar Alum, Kyle Kraft, Morteza Abbaszadegan

41

Whole genome sequencing reveals prophage sequences present in Escherichia coli isolated from pristine environments

– Alfredo Gonzalez

42

Chemical Induction Followed by High-Throughput Sequencing: Prophage Presence, Host Range and Promiscuity as Possible Roles in Genome Evolution

– Yadeliz Adlin, Serrano Matos